Cost-effective biodiversity monitoring for EU policies

Overview

Horizon Europe Research and Innovation Action HORIZON-NATURE funds cost-effective, scalable biodiversity monitoring to support implementation of the Nature Restoration Regulation (pollinators), the Soil Monitoring Law (soil biodiversity) or the Water Framework Directive (freshwater biological elements). The single-stage lump-sum call opens 13 October 2026 with deadline 14 January 2027, has an indicative budget of €16 million and expects to fund around three projects with €5–€6 millioneach. Proposals must select one area (A, B or C), demonstrate operational pilots with end-user engagement, advance DNA/genomic reference libraries and markers, ensure FAIR-by-design data and plan cluster-level joint activities and policy engagement. Eligible applicants follow Horizon Europe rules and consortia should combine ecology, genomics, bioinformatics, data stewardship, AI and competent authorities to deliver operational monitoring tools.

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Highlights

What it funds

Scope summary

Research and innovation actions to develop, test and pilot scalable, cost-effective biodiversity monitoring approaches that support implementation of EU environmental legislation (Nature Restoration Regulation, Soil Monitoring Law, Water Framework Directive). Projects must demonstrate operational monitoring cases, advance genomic/DNA methods and libraries, ensure FAIR data and plan cluster-level joint activities.

Eligible topics (indicate A, B or C on application):Area A Pollinators (NRR); Area B Soil biodiversity (SML); Area C Freshwater biological elements (WFD). Proposals must clearly state which area they target and may integrate technologies (eDNA, genomics, AI, automated monitoring) and end-user engagement 1.

  1. 1Develop and pilot scalable, cost‑effective monitoring methods and demonstration cases
  2. 2Advance and complete DNA/genomic reference libraries and markers for target taxa per area
  3. 3Integrate multiple technologies (e.g., eDNA/metabarcoding, sensors, AI) and engage competent authorities
  4. 4Ensure data are FAIR and explore use of EOSC and research infrastructures (e.g., eLTER)
  5. 5Participate in a funded-project cluster and allocate a work package to joint networking and policy engagement
AreaExample required DNA library targets
A Nature Restoration Regulation (Pollinators)Bees, hoverflies, butterflies and moths; advance other pollinating insect groups
B Soil Monitoring Law (Soil biodiversity)Main fungi and bacteria groups; progress libraries for archaea, protists and other soil organisms
C Water Framework Directive (Freshwater)Biological quality elements: fish, macroinvertebrates, phytobenthos (diatoms), phytoplankton, macrophytes, macroalgae, angiosperms

Type of action HORIZON Research and Innovation Actions (RIA). Single-stage call; submission opens 13 October 2026; deadline 14 January 2027 17:00 Brussels time. Lump-sum grants apply.

Budget and expected grant size:Topic budget €16,000,000 (2027). Indicative number of grants 3; expected EU contribution per project €5,000,000 to 6,000,000.

  1. 1Who can apply: legal entities from Horizon Europe eligible Member States and Associated Countries (see Work Programme Annex B for specifics)
  2. 2Consortium expectations: multi‑partner consortia with end‑user involvement (competent authorities, monitoring agencies); size and composition must be justified
  3. 3Evaluation: standard Horizon Europe RIA award criteria; selection ensures coverage across Areas A, B and C where possible

Additional procedural notes:proposals must respect page limits and templates in the Submission System, may be required to use Copernicus/Galileo services if EO data are used, and must allocate budget and a work package for cluster-level coordination and policy engagement.

Footnotes

  1. 1Full topic text and submission details are available on the Funding & Tenders Portal Topic page.

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Breakdown

Call summary and objectives

This is a Horizon Europe Research and Innovation Actions (HORIZON-RIA) call under the destination Transformative change towards a nature-positive economy and society. The topic title is Cost-effective biodiversity monitoring for EU policies HORIZON-NATURE. The call opens on 13 October 2026 and the deadline is 14 January 2027, single-stage, with submission through the EU Funding & Tenders Portal Submission System. The action will be implemented under a lump-sum model (HORIZON-AG-LS).

Primary expected outcomes:More precise and cost-effective biodiversity monitoring schemes built on advanced technologies and increased data; improved user-friendly tools for policymakers and competent authorities to implement and evaluate EU environmental legislation, leading to simpler policy implementation and better ecosystem protection and restoration.

Scope and required focus areas

Proposals must address one clearly indicated Area:A Nature Restoration Regulation (NRR) focused on the EU Pollinators Monitoring Scheme; B Soil Biodiversity for the Soil Monitoring Law (SML); or C Water Framework Directive (WFD). All proposals, irrespective of area, must develop scalable, cost-effective approaches for legally binding biodiversity monitoring, integrate synergistic technologies and methods, engage end-users, produce demonstration cases for operationalisation and piloting under the relevant EU legislation, assess scalability and limitations, advance DNA-based and genomic techniques for species identification and abundance monitoring, and where possible develop automated and real-time AI-supported monitoring frameworks. Proposals must build on previous and ongoing relevant projects/initiatives (e.g., EuropaBON, MAMBO, BGE, Biodiversa+, A Soil Deal for Europe, EBOCC) and ensure data is FAIR-by-design and interoperable with EOSC and relevant research infrastructures (e.g., eLTER). All selected projects will be required to form a cluster and fund a dedicated work package for joint networking, data and policy strategies, joint dissemination, thematic trainings and cooperation with the EC Knowledge Centre for Biodiversity (KCBD).

Area-specific DNA library requirements:Area A must complete DNA libraries for key pollinator groups (bees, hoverflies, butterflies and moths) and substantially advance libraries for other pollinating insect groups. Area B must complete DNA libraries for main fungi and bacteria groups and substantially advance libraries for archaea, protists and other soil organisms. Area C must complete DNA libraries for WFD biological quality elements (fish, macroinvertebrates, phytobenthos including diatoms, phytoplankton, macrophytes, macroalgae, angiosperms). Proposals should also develop additional markers where standard barcoding markers do not suffice for species-level identification.

Administrative and procedural conditions

Type of action:HORIZON Research and Innovation Actions (RIA). Type of Model Grant Agreement: HORIZON Lump Sum Grant (HORIZON-AG-LS). Deadline model: single-stage. Planned opening date: 13 October 2026; Deadline date and time: 14 January 2027, 17:00 Brussels time. Submission via the EU Funding & Tenders Portal Submission System using the standard application form for HE RIA/IA (Part A and Part B templates available in the Submission System/Reference Documents). Proposal page limits and layout: defined in Part B of the Application Form and Annexes A and E of the Work Programme General Annexes. Evaluation and award procedures follow Horizon Europe General Annexes D and F, with topic-specific portfolio balancing to ensure funding across the three Areas A, B and C where thresholds are met.

Budget and indicative grant size:Total topic budget (2027): €16,000,000. Expected EU contribution per project: between €5,000,000 and €6,000,000. Indicative number of grants: 3.

  1. 1Planned opening date: 13 October 2026
  2. 2Deadline: 14 January 2027 17:00:00 Brussels time
  3. 3Single-stage submission
  4. 4Lump sum grant model

Eligibility and participation

Eligible countries follow Horizon Europe Annex B (Member States and Associated Countries). Some non-associated third countries may participate under specific national arrangements as described in Annex B and the Horizon Europe Programme Guide. General eligibility conditions, financial and operational capacity and exclusion rules are described in General Annexes B and C of the Horizon Europe Work Programme. If projects use satellite-based Earth observation or GNSS services, beneficiaries must use Copernicus and/or Galileo/EGNOS. Proposers must follow any restrictions on participation described in General Annex B (including ownership/control restrictions for certain critical technology areas as specified in related topics).

Eligible applicant types:The call is open to legal entities that meet Horizon Europe eligibility: universities and higher education establishments, public research organisations and research institutes, SMEs and large enterprises, non-profit organisations and NGOs, public authorities, and other legal entities established in eligible Member States and Associated Countries. Consortia are standard for RIA but single legal entities may participate if consistent with the topic eligibility and expected impact; consortium composition should be justified and industry/end-user engagement demonstrated.

Funding modality, co-funding and financial setup

Funding type:grant in the form of lump-sum contributions under Horizon Europe (see the Decision authorising the use of lump sums). Eligible costs are covered by a lump sum contribution defined in the lump-sum decisions and related guidance; see the Lump Sums Decision and Annex G of the General Annexes for details Decision on lump sums. Co-funding: for lump-sum actions Horizon Europe typically does not require a fixed co-funding percentage but applicants must present a realistic budget and work plan compatible with the lump-sum funding and deliverables; proposals should describe additional resources (private, national co-funding or in-kind) if relevant to ensure delivery and sustainability. Financial capacity and exclusion criteria apply as in Annex C of the Work Programme.

Call elementDetail
ProgrammeHorizon Europe (HORIZON)
Call identifierHORIZON-NATURE
Type of actionHORIZON-RIA (Research & Innovation Action)
Type of MGAHORIZON Lump Sum Grant (HORIZON-AG-LS)
Opening date13 October 2026
Deadline14 January 2027, 17:00 Brussels time
Submission procedureSingle-stage
Topic total budget (2027)€16,000,000
Expected EU contribution/project€5,000,000 to 6,000,000
Indicative number of grants3

Evaluation, award and selection specificities

Evaluation criteria and scoring follow Horizon Europe General Annex D and the standard evaluation form for HE RIA/IA, with topic-specific adaptations. Thresholds for individual criteria and overall pass thresholds are applied as in General Annex D. To ensure balanced coverage, the granting authority will allocate grants not only by ranking but also to the highest ranked proposals per area (A, B or C) subject to budget availability and attainment of thresholds. Evaluation and award processes and indicative timeline follow General Annex F and the Online Manual.

Application stages and process:Single-stage submission through the EU Funding & Tenders Portal. Applicants must complete Part A (administrative data) and Part B (technical proposal) using the application form available in the Submission System. Page limits and layout are defined in the application form (Part B) and Annexes A and E. No separate stage(s) are foreseen in this topic; evaluation proceeds after the single-stage submission.

Number of application stages

1 stage (single-stage call).

Success rates

The call text does not provide explicit historical success rates. Three grants are indicative for this topic with an expected EU contribution of €5-€6 millioneach and a topic budget of €16 million. Actual success rates will depend on the number and quality of proposals received.

Technical and scientific expectations

Technical expectations are high:proposals must develop and pilot scalable, cost-effective monitoring approaches aligned with legally binding monitoring needs under NRR, SML or WFD; integrate multiple technologies (e.g., in-situ sensors, remote sensing, eDNA/metabarcoding, genomic techniques, AI for automated identification and real-time analysis); produce demonstration cases and operational workflows; complete or substantially advance DNA reference libraries and markers for target taxa; assess scalability, gaps and limitations; ensure data are FAIR-by-design and interoperable with EOSC and research infrastructures (e.g., eLTER); include user-driven co-design with end-users, policymakers and competent authorities; and allocate a dedicated work package and budget for cluster activities with other funded projects and the EC Knowledge Centre for Biodiversity (KCBD).

  1. 1Integrate DNA-based methods (eDNA, metabarcoding) and advance genomic techniques for abundance monitoring and indicator integration
  2. 2Complete or substantially advance DNA libraries for the taxa indicated per Area (A, B, C) and develop additional markers if necessary
  3. 3Develop demonstration and pilot cases for operationalisation and legal monitoring requirements with end-user engagement
  4. 4Develop automated and real-time monitoring frameworks with AI where possible and assess limitations and scalability
  5. 5Ensure data FAIRness and interoperability; consider EOSC and research infrastructures such as eLTER
  6. 6Form a project cluster with other funded projects and allocate dedicated budget for joint activities, data management and policy engagement

Target project maturity and TRL

Projects are expected to operate at applied research and development TRLs suitable for demonstration and piloting within the legislative context. As an RIA, work can range from development and validation of novel methods up to demonstration in operational contexts; proposals should explain TRL progression and the pathway to integration in monitoring frameworks.

Administrative templates, forms and supporting documents

Applicants must use the application form available in the Submission System (standard HE RIA/IA templates). Evaluation form templates (HE RIA/IA) will be used with necessary adaptations. Key reference documents: HE Main Work Programme 2026-2027, General Annexes (A to G), Guidance on Lump Sums, Model Lump Sum MGA, Rules for Legal Entity Validation, LEAR Appointment and Financial Capacity Assessment, Funding & Tenders Portal Online Manual and Terms and Conditions. Call-specific instructions include the Detailed Budget Table for lump-sum grants and guidance 'Lump sums - what do I need to know?'.

Application form structure and recommended content:Use the Submission System Part B template. Ensure inclusion of: project summary and impact statement; description of objectives, methodology and work packages; demonstration and pilot plan with sites, partners and end-user engagement; technical description of methods/technologies (e.g., eDNA sampling, sequencing workflow, bioinformatics, AI models, sensor specifications); data management plan addressing FAIR-by-design and EOSC interoperability; DNA library development plan with markers and taxonomic coverage; risk assessment and go/no-go decision points; project management and consortium roles; detailed deliverables and milestones; exploitation, dissemination and clustering work package including budget allocation for joint network activities and policy engagement with KCBD; and a realistic lump-sum budget justification aligned to deliverables.

Consortium recommendations

While the topic does not prescribe a minimum number of partners beyond general Horizon Europe rules, consortia should include multidisciplinary partners: taxonomy/systematics specialists, molecular ecology/genomics labs, bioinformatics and AI specialists, sensor and remote sensing experts, data managers familiar with FAIR and EOSC integration, national monitoring bodies or competent authorities (end-users), and stakeholders for piloting (e.g., river basin authorities, agricultural authorities, conservation agencies). The consortium must demonstrate the ability to collaborate with existing initiatives (e.g., EBOCC, eLTER) and to contribute to the project cluster activities.

Beneficiary geographic scope

Geographic eligibility follows Horizon Europe:applicants established in EU Member States and Horizon Europe Associated Countries are eligible. Certain non-associated third countries may participate under specific conditions described in Annex B and the Horizon Europe Programme Guide; National Contact Points provide country-specific guidance.

Mentioned countries and regions

No specific Member State list is named in the topic text; eligibility defaults to EU Member States and Horizon Europe Associated Countries as per Annex B. The topic references EU legislation and EU-level bodies and infrastructures (e.g., NRR, SML, WFD, CAP, EOSC, eLTER, KCBD).

Support, help and partner search

Support resources:Funding & Tenders Portal Online Manual, Horizon Europe Programme Guide, National Contact Points, Enterprise Europe Network, IT Helpdesk, European IPR Helpdesk, CEN-CENELEC and ETSI Research Helpdesks, and Partner Search tools on the Funding & Tenders Portal. The portal allows publication of partner search announcements for this topic.

Templates and annexes referenced:Applicants must use the Part A and Part B application form in the Submission System. Example templates and the standard evaluation form are available in the Reference Documents page of the Funding & Tenders Portal. The Lump Sum MGA and guidance on lump sums must be consulted for financial setup Lump Sum Decision.

Risk factors and assessment criteria applicants should address

  1. 1Feasibility and reliability of DNA-based markers for accurate species-level identification across target taxa and geographic ranges
  2. 2Scalability and cost-effectiveness of sampling and laboratory workflows for national monitoring schemes
  3. 3Interoperability and FAIRness of data pipelines and integration with national monitoring databases and EOSC
  4. 4Accuracy, bias and robustness of AI-supported automated identification systems and steps to ensure rigorous validation
  5. 5Legal and procedural readiness for integration into binding monitoring frameworks (per NRR, SML, WFD) and risk of non-acceptance by competent authorities
  6. 6Sustainability and long-term maintenance of reference DNA libraries and data repositories

Practical recommendations for applicants

Demonstrate strong end-user engagement and co-design with competent authorities; allocate sufficient resources to build/complete DNA reference libraries and marker development; provide clear demonstration and piloting protocols and sites; include a robust Data Management Plan ensuring FAIR-by-design; plan for cluster participation and joint activities; explain how outputs will be integrated into policy-relevant monitoring frameworks and provide a realistic timeline with go/no-go decision points for operational pilots.

Footnotes

  1. 1Lump-sum decision and related guidance available on Funding & Tenders Portal: ec.europa.eu

Short Summary

Impact

Deliver scalable, cost‑effective biodiversity monitoring methods and operational tools that provide more precise, actionable, FAIR data to support implementation and evaluation of EU environmental legislation and improve ecosystem protection and restoration.

Applicant

Teams able to integrate ecology, genomics (eDNA/metabarcoding/genomics), bioinformatics, AI/automated monitoring, data stewardship (FAIR/EOSC), and policy implementation expertise to co‑design and validate monitoring in real operational settings.

Developments

Development, validation and piloting of scalable monitoring approaches and DNA/genomic reference libraries for one of: pollinators (NRR), soil biodiversity (SML), or freshwater biological quality elements (WFD), including demonstration cases, markers and automated/AI workflows.

Applicant Type

Researchers and research organisations, public authorities and monitoring agencies, specialist SMEs and technology/data providers, and non‑profit/environmental organisations with relevant technical abilities.

Consortium

Designed for multidisciplinary consortia combining scientific, technical and end‑user monitoring/policy expertise rather than single standalone applicants.

Funding Amount

Indicative EU contribution per project €5,000,000 to €6,000,000 (topic budget €16,000,000; around 3 grants).

Countries

Eligible entities established in EU Member States and Horizon Europe Associated Countries are relevant because the call supports implementation of EU legislation (NRR, SML, WFD).

Industry

Horizon Europe – Transformative change towards a nature‑positive economy and society (biodiversity monitoring / environmental policy implementation).

Additional Web Data

This topic is a Horizon Europe Research and Innovation Action under the call Transformative change towards a nature positive economy and society, and it supports the work programme ambition to strengthen biodiversity evidence, policy implementation, and the EU biodiversity agenda through more actionable monitoring data.315 The call is designed to move promising biodiversity monitoring methods from pilot stage into operational use for EU law, with a strong emphasis on scalable, cost effective, FAIR by design data generation and better tools for policymakers and competent authorities.3

FieldDetails
Programme and callHorizon Europe, Transformative change towards a nature positive economy and society, topic HORIZON NATURE 2027 02 05.315
Type of actionHORIZON RIA, Research and Innovation Action.3
Grant modelLump sum grant.3
Submission modelSingle stage and forthcoming.3
Planned opening13 October 2026.39
Deadline14 January 2027 at 17:00 Brussels time.39
Indicative budget€16 million total.3
Expected EU contribution per project€5 million to €6 million.34
Indicative number of grantsAround 3 projects.34

What the funding is about

The topic addresses the gap between advanced biodiversity monitoring technologies and their use in binding EU monitoring schemes. It focuses on the Nature Restoration Regulation and the EU Pollinators Monitoring Scheme, the Soil Monitoring Law and soil biodiversity monitoring, and the Water Framework Directive and freshwater biodiversity monitoring.3 Proposals are expected to improve the cost effectiveness of legally binding monitoring, test demonstration cases in real implementation settings, assess the scalability and limitations of approaches, and advance species identification and quantification methods including DNA based and genomic techniques.3

Topic areas and required focus

AreaPolicy focusExpected scientific and technical emphasis
Area ANature Restoration Regulation and the EU Pollinators Monitoring SchemeComplete DNA libraries for key pollinators such as bees, hoverflies, butterflies and moths, and substantially advance coverage for other relevant pollinating insects.3
Area BSoil Monitoring Law and soil biodiversityComplete DNA libraries for the main groups of fungi and bacteria and substantially advance libraries for archaea, protists and other relevant soil organisms.3
Area CWater Framework Directive and freshwater ecosystemsComplete DNA libraries for biological quality elements such as fish, macroinvertebrates, phytobenthos including diatoms, phytoplankton, macrophytes, macroalgae, and angiosperms.3

Who can apply and what a strong consortium looks like

Eligibility follows the general Horizon Europe rules in the work programme annexes, and the topic is best suited to consortia that can combine ecology, genomics, bioinformatics, data stewardship, AI, and policy implementation expertise.3 In practice, successful applicants are likely to include research organisations, universities, public authorities, environmental agencies, specialist SMEs, data and technology providers, and end users who can validate the methods in operational monitoring contexts.3

  • Choose one area, A, B or C, and state it clearly in the application.3
  • Design the project around scalable monitoring approaches that integrate complementary technologies and methods, with end user involvement from the start.3
  • Include demonstration cases that test, operationalise, and pilot the monitoring approach in a realistic implementation setting.3
  • Where possible, include automated and real time monitoring supported by AI.3
  • Compare the approaches on scalability, gaps, limitations, and possible drawbacks.3
  • Advance DNA libraries, markers, species identification, quantification, and genomic techniques for the selected policy area.3
  • Build on earlier and ongoing initiatives such as EuropaBON, MAMBO, BGE, Biodiversa+, the Soil mission, and the European Biodiversity Observation and Coordination Centre.3
  • Provide FAIR by design data workflows and consider the European Open Science Cloud and relevant research infrastructures such as eLTER where relevant.3
  • Include a dedicated work package and sufficient budget for cluster level networking, joint dissemination, joint data management, policy outputs, workshops, and cooperation with the EC Knowledge Centre for Biodiversity.3

Funding, format, and timeline

The call is currently forthcoming, with the submission system planned to open on 13 October 2026 and the deadline set for 14 January 2027 at 17:00 Brussels time.39 The budget envelope for this topic is €16 million, and the Commission expects about 3 grants, with each project typically receiving between €5 million and €6 million under the lump sum model.34 Because it is a lump sum grant, applicants should make sure that work packages, outputs, and internal milestones are clearly structured and internally consistent with the proposed lump sum budget.3

Evaluation and selection points applicants should note

  • The Commission will seek a balanced portfolio across areas A, B, and C, and can award funding not only by overall ranking but also to the highest ranked proposal in each area, subject to budget availability and all thresholds being met.3
  • If satellite based earth observation, positioning, navigation, or related timing data are used, beneficiaries must make use of Copernicus and or Galileo EGNOS, although other data and services may also be used.3
  • General Horizon Europe rules apply for admissibility, eligible countries, financial and operational capacity, exclusion criteria, and grant set up.3
  • Projects should form a cluster and participate in common networking and joint activities, including a shared dissemination strategy, shared data management and policy strategy, thematic workshops, and cooperation with the EC Knowledge Centre for Biodiversity.3

Practical applicant takeaway

This opportunity is most attractive for consortia that can turn frontier biodiversity monitoring science into operational public policy tools. The strongest proposals will show a credible route to real world use, robust data governance, clear collaboration with policy and monitoring authorities, and a convincing plan to deliver better biodiversity evidence at lower cost.315

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